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Assume you inoculated 100 cells, with a generation time of 20 minutes, into 100 ml of nutrient broth. You then inoculated 100 cells of the same species into 200 ml of nutrient broth. After incubation for 4 hours, you can reasonably expect to have


A) more cells in the 100 ml.
B) more cells in the 200 ml.
C) the same number of cells in both.
D) The answer cannot be determined based on the information provided.

E) B) and D)
F) All of the above

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Consider a culture medium on which only gram-positive organisms such as Staphylococcus aureus colonies can grow due to an elevated NaCl level. A yellow halo surrounds the growth, indicating the bacterium fermented a sugar in the medium, decreasing the pH as a result and changing the color of a pH indicator chemical. This type of medium would be referred to as a(n)


A) selective and differential medium.
B) differential medium.
C) selective medium.
D) enrichment culture.
E) differential and enrichment culture.

F) A) and B)
G) A) and C)

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Which of the following is an advantage of the direct microscopic count?


A) sample volume is unknown
B) requires no incubation time
C) can easily distinguish live from dead cells
D) can readily count organisms that are motile
E) requires a large number of cells

F) C) and D)
G) D) and E)

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Which group of microorganisms is most likely to spoil a freshwater trout preserved with salt?


A) hyperthermophiles
B) psychrophiles
C) facultative halophiles
D) anaerobes
E) thermophiles

F) A) and E)
G) B) and C)

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An experiment began with 4 cells and ended with 128 cells. How many generations did the cells go through?


A) 5
B) 6
C) 32
D) 64
E) 4

F) A) and B)
G) B) and E)

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Figure 6.3 Figure 6.3    -In Figure 6.3, which tube shows the expected growth pattern for a microaerophile? A)  a B)  b C)  c D)  d E)  e -In Figure 6.3, which tube shows the expected growth pattern for a microaerophile?


A) a
B) b
C) c
D) d
E) e

F) C) and D)
G) A) and E)

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Figure 6.3 Figure 6.3    -In Figure 6.3, which tube shows the expected growth pattern for a facultative anaerobe? A)  a B)  b C)  c D)  d E)  e -In Figure 6.3, which tube shows the expected growth pattern for a facultative anaerobe?


A) a
B) b
C) c
D) d
E) e

F) All of the above
G) B) and D)

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Which enzyme catalyzes the reaction: H2o2 + 2H+ → 2H2O?


A) catalase
B) oxidase
C) peroxidase
D) superoxide dismutase

E) B) and D)
F) All of the above

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Patients with indwelling catheters (long-term tubes inserted into body orifices for drainage, such as through the urethra and into the urinary bladder) are susceptible to infections because


A) bacteria cause infections.
B) injected solutions are contaminated.
C) infections can be transmitted from other people.
D) their immune systems are weakened.
E) biofilms develop on catheters.

F) B) and C)
G) B) and E)

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The term aerotolerant anaerobe refers to an organism that


A) tolerates normal atmospheric nitrogen gas levels.
B) requires less oxygen than is present in air.
C) does not use oxygen but tolerates it.
D) is killed by oxygen.
E) requires more oxygen than is present in air.

F) A) and B)
G) B) and E)

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Most bacteria reproduce by


A) budding.
B) binary fission.
C) aerial hyphae.
D) fragmentation.
E) mitosis.

F) A) and B)
G) C) and D)

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Figure 6.2 Figure 6.2    -Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture. In the figure, which sections of the graph illustrate a logarithmic change in cell numbers? A)  a and b B)  a and d C)  c and d D)  b and d E)  a and c -Figure 6.2 shows a typical bacterial growth curve with the y-axis indicating the log of the number of bacteria and the x-axis indicating time in culture. In the figure, which sections of the graph illustrate a logarithmic change in cell numbers?


A) a and b
B) a and d
C) c and d
D) b and d
E) a and c

F) C) and E)
G) B) and C)

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