Question 1

Match the description to the most likely identification.
-Fusobacterium spp.

Accepted Answer

A)  Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile
B)  bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative
C)  fluoresce brick red or brown to black pigment
D)  reduces nitrate and requires formate and fumarate for growth in broth culture
E)  sensitive to kanamycin and fluoresce a chartreuse colorF) C) and E)
G) C) and D)

Question 2

The most likely identification of an anaerobic gram-positive bacillus that is a very large fusiform bacillus with one pointed end and one blunt end is

Accepted Answer

A)  Eubacterium.
B)  Veillonella.
C)  Leptotrichia.
D)  Propionibacterium.
E)  Lactobacillus.F) A) and D)
G) All of the above

Question 3

The media used for isolating the anaerobic agent of bacteremia may include

Accepted Answer

A)  thioglycollate.
B)  Schaedler's broth.
C)  thiol broth.
D)  None of the above are correct.
E)  All of the above are correct.F) A) and B)
G) A) and C)

Question 4

The absence of leukocytes in a Gram stain of a clinical specimen

Accepted Answer

A)  rules out the presence of a serious anaerobic infection.
B)  may indicate that the causative organism produces necrotizing toxins.
C)  may indicate poor transport methods.
D)  may indicate excessive exposure to air during specimen processing.E) None of the above
F) B) and C)

Question 5

The media used for culturing a blood specimen for anaerobes may include

Accepted Answer

A)  anaerobic blood agar.
B)  Schaedler's broth.
C)  5% sheep blood agar.
D)  laked kanamycin-vancomycin blood agar.
E)  All of the above are correct.F) A) and B)
G) B) and D)

Question 6

The most likely identification of a bile resistant, kanamycin, colistin, and vancomycin-resistant anaerobic gram-negative bacilli is

Accepted Answer

A)  nonpigmented Prevotella.
B)  Porphyromonas spp.
C)  Bacteroides fragilis group.
D)  Fusobacterium spp.
E)  Bacteroides ureolyticus.F) A) and C)
G) A) and E)

Question 7

The primary plating media for inoculating a wound aspirate for anaerobic culture may include

Accepted Answer

A)  anaerobic blood agar.
B)  laked kanamycin-vancomycin blood agar.
C)  aerobic 5% sheep blood agar.
D)  thioglycollate.
E)  All of the above are correct.F) B) and D)
G) C) and E) E

Question 8

Match the description to the most likely identification.
-Nonpigmented Prevotella

Accepted Answer

A)  Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile
B)  bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative
C)  fluoresce brick red or brown to black pigment
D)  reduces nitrate and requires formate and fumarate for growth in broth culture
E)  sensitive to kanamycin and fluoresce a chartreuse colorF) All of the above
G) C) and D)

Question 9

Incubation conditions for anaerobic cultures include

Accepted Answer

A)  30° to 35° C.
B)  incubation for 48 hours before exposing to air.
C)  incubated for at least 3 days before discarding.
D)  incubation of broth medium aerobically with the cap loose.
E)  All of the above are correct.F) A) and B)
G) B) and E)

Question 10

The most likely identification of an anaerobic gram-negative bacilli that will fluoresce brick red or has brown to black pigment is

Accepted Answer

A)  nonpigmented Prevotella.
B)  Porphyromonas spp.
C)  Bacteroides fragilis group.
D)  Fusobacterium spp.
E)  Bacteroides ureolyticusF) B) and E)
G) D) and E) B

Question 11

Match the description to the most likely identification.
-Bacteroides fragilis group

Accepted Answer

A)  Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile
B)  bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative
C)  fluoresce brick red or brown to black pigment
D)  reduces nitrate and requires formate and fumarate for growth in broth culture
E)  sensitive to kanamycin and fluoresce a chartreuse colorF) B) and E)
G) B) and D)

Question 12

The most likely identification of an anaerobic, curved, gram-variable bacillus with tapered ends on Gram stain of vaginal secretions when vaginosis is suspected is

Accepted Answer

A)  Porphyromonas spp.
B)  Actinomyces.
C)  Mobiluncus
D)  Fusobacterium spp.
E)  Bifidobacterium.F) All of the above
G) A) and C)

Question 13

Characteristics that strongly indicate the presence of anaerobes in a clinical specimen include

Accepted Answer

A)  a foul odor.
B)  sulfur granules.
C)  brick-red fluorescence under long-wavelength ultraviolet (UV)  light.
D)  All of the above are correct.E) B) and D)
F) None of the above

Question 14

Match the description to the most likely identification.
-Bacteroides ureolyticus

Accepted Answer

A)  Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile
B)  bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative
C)  fluoresce brick red or brown to black pigment
D)  reduces nitrate and requires formate and fumarate for growth in broth culture
E)  sensitive to kanamycin and fluoresce a chartreuse colorF) A) and D)
G) B) and C)

Question 15

Match the description to the most likely identification.
-Pigmented Prevotella

Accepted Answer

A)  Kanamycin, colistin, and vancomycin-resistant and grow in 20% bile
B)  bile-sensitive, kanamycin-resistant, catalase-negative and indole-negative
C)  fluoresce brick red or brown to black pigment
D)  reduces nitrate and requires formate and fumarate for growth in broth culture
E)  sensitive to kanamycin and fluoresce a chartreuse colorF) A) and D)
G) A) and C)

Question 16

The most likely identification of an anaerobic Gram negative bacilli that reduces nitrate and requires formate and fumarate for growth in broth culture is

Accepted Answer

A)  Porphyromonas spp.
B)  Bacteroides fragilis group.
C)  pigmented Prevotella.
D)  Fusobacterium spp.
E)  Bacteroides ureolyticus.F) A) and D)
G) B) and E)

Question 17

Choose the statement that is most true regarding the identification of anaerobes.

Accepted Answer

A)  Complete identification can be very costly.
B)  Complete identification often requires several different testing methods.
C)  Most clinical laboratories no longer perform complete identification of anaerobes.
D)  Presumptive identification is often just as useful as complete identification.
E)  All of the above are correct.F) All of the above
G) C) and E) E

Question 18

The most likely identification of kanamycin-sensitive, anaerobic, gram-negative bacilli that will fluoresce a chartreuse color is

Accepted Answer

A)  Fusobacterium spp.
B)  nonpigmented Prevotella.
C)  Bacteroides ureolyticus.
D)  Porphyromonas spp.
E)  pigmented Prevotella.F) A) and D)
G) A) and C)

Question 19

The ethanol shock or heat shock test will separate which two bacteria?

Accepted Answer

A)  Clostridium perfringens and Clostridium ramosum
B)  Clostridium and Bifidobacterium
C)  Actinomyces and Bifidobacterium
D)  Eubacterium and EggerthellaE) B) and C)
F) None of the above

Question 20

Fecal cultures for isolation of Clostridium difficile are plated on

Accepted Answer

A)  anaerobic blood agar.
B)  laked kanamycin-vancomycin blood agar.
C)  aerobic 5% sheep blood agar.
D)  cycloserine cefoxitin fructose agar.
E)  All of the above are correct.F) B) and E)
G) A) and B)

Exam 41: Laboratory Considerations

Match the description to the most likely identification. -Fusobacterium spp.

Correct AnswerverifiedShow Answer

The most likely identification of an anaerobic gram-positive bacillus that is a very large fusiform bacillus with one pointed end and one blunt end is

Correct AnswerverifiedShow Answer

The media used for isolating the anaerobic agent of bacteremia may include

Correct AnswerverifiedShow Answer

The absence of leukocytes in a Gram stain of a clinical specimen

Correct AnswerverifiedShow Answer

The media used for culturing a blood specimen for anaerobes may include

Correct AnswerverifiedShow Answer

The most likely identification of a bile resistant, kanamycin, colistin, and vancomycin-resistant anaerobic gram-negative bacilli is

Correct AnswerverifiedShow Answer

The primary plating media for inoculating a wound aspirate for anaerobic culture may include

Correct AnswerverifiedE

Match the description to the most likely identification. -Nonpigmented Prevotella

Correct AnswerverifiedShow Answer

Incubation conditions for anaerobic cultures include

Correct AnswerverifiedShow Answer

The most likely identification of an anaerobic gram-negative bacilli that will fluoresce brick red or has brown to black pigment is

Correct AnswerverifiedB

Match the description to the most likely identification. -Bacteroides fragilis group

Correct AnswerverifiedShow Answer

The most likely identification of an anaerobic, curved, gram-variable bacillus with tapered ends on Gram stain of vaginal secretions when vaginosis is suspected is

Correct AnswerverifiedShow Answer

Characteristics that strongly indicate the presence of anaerobes in a clinical specimen include

Correct AnswerverifiedShow Answer

Match the description to the most likely identification. -Bacteroides ureolyticus

Correct AnswerverifiedShow Answer

Match the description to the most likely identification. -Pigmented Prevotella

Correct AnswerverifiedShow Answer

The most likely identification of an anaerobic Gram negative bacilli that reduces nitrate and requires formate and fumarate for growth in broth culture is

Correct AnswerverifiedShow Answer

Choose the statement that is most true regarding the identification of anaerobes.

Correct AnswerverifiedE

The most likely identification of kanamycin-sensitive, anaerobic, gram-negative bacilli that will fluoresce a chartreuse color is

Correct AnswerverifiedShow Answer

The ethanol shock or heat shock test will separate which two bacteria?

Correct AnswerverifiedShow Answer

Fecal cultures for isolation of Clostridium difficile are plated on

Correct AnswerverifiedShow Answer

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E

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B

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E

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