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Which is false regarding the two-component regulatory system in bacteria?


A) The system involves a sensor kinase and a response regulator.
B) The sensor kinase is phosphorylated by the response regulator when the extracellular environment changes.
C) Regulation of OmpF and OmpC porin proteins in
D) One part of the system is exposed to the external cellular environment while another part is exposed to the cytoplasm.
E) coli is modulated by this type of system.

F) C) and E)
G) A) and C)

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In terms of global gene regulation,which of the following molecules is most similar to c-di-GMP and cAMP?


A) ppGpp
B) rRNA
C) ATP
D) EnvZ

E) A) and B)
F) None of the above

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A

In which situation might methylation of membrane proteins in Escherichia coli initiate a phosphorelay system that evokes clockwise rotation of flagella?


A) Presence of a chemorepellent in the extracellular environment.
B) Change in environmental osmotic pressure.
C) Utilization of lactose due during diauxic growth.
D) Cellular population reaches a quorum.

E) A) and B)
F) None of the above

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If a set of genes is repressed in the presence of the controlling substance,it is referred to as __________ control.

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What term describes a set of genes that is controlled by a common regulatory protein?


A) Codon
B) Regulon
C) Transcription
D) Kinase

E) B) and C)
F) A) and D)

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Which of the following is an example of gene regulation at the posttranslational level?


A) Alteration of protein structure
B) Early termination of an mRNA transcript
C) Initiation of protein synthesis
D) Prevention of RNA synthesis

E) A) and B)
F) A) and C)

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Guanine tetraphosphate (ppGpp) is particularly associated with which process?


A) Spore formation in Bacillus
B) Chemiosmosis in Paracoccus
C) Stringent response in Escherichia
D) Quorum sensing in Vibrio

E) C) and D)
F) A) and D)

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Two-component signal transduction systems are found in the Bacteria,but not in Archaea.

A) True
B) False

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Acylhomoserine lactones used in quorum sensing regulate their own synthesis by a(n) ____________ system.


A) riboswitch
B) antisense
C) catabolite repression
D) autoinduction

E) C) and D)
F) B) and D)

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D

The


A) one
B) two
C) three
D) four
E) coli lac operon has ________ operator sequences.

F) None of the above
G) B) and C)

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If autophosphorylation of CheA protein does not occur,then Escherichia coli cells must be


A) in a gradient of a chemoattractant.
B) in a gradient of a chemorepellant.
C) tumbling.
D) rotating flagella clockwise.

E) C) and D)
F) None of the above

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In catabolite repression systems of some Gram-positive bacteria,such as Bacillus subtilis,transcription of catabolic operons is regulated by CcpaA (catabolite control protein A) rather than cAMP.Propose a hypothesis to explain the preferential use of glucose in the presence of a second sugar such as sucrose by Bacillus subtilis.


A) CcpaA acts as a repressor of sucrose operon genes in Bacillus subtilis when both sugars are present.
B) CcpaA acts as an inducer of sucrose operon genes in Bacillus subtilis when both sugars are present.
C) CcpaA acts as a repressor of sucrose operon genes in Bacillus subtilis when lactose,but not glucose,is present.
D) CcpaA acts as an inducer of sucrose operon genes in Bacillus subtilis when glucose,but not lactose,is present.

E) All of the above
F) A) and B)

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The activity of adenyl cyclase is influenced by


A) the phosphotransferase system.
B) the presence of lactose in the environment.
C) homoserine lactones.
D) None of the choices are correct.

E) A) and B)
F) A) and D)

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A

Scientists first focused on gene regulation at the level of translation,rather than transcription,because more was known about protein than nucleic acids at the time.

A) True
B) False

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In some circumstances,when two different carbon sources are available,growth will occur first using one carbon source,then after a short lag period,growth will resume using the second carbon source.This process is called __________ growth.


A) alternating
B) bimorphic
C) diauxic
D) dimorphic

E) A) and B)
F) B) and D)

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During the early stages of sporulation in Bacillus subtilis,________ is active in the forespore and ________ is active in the mother cell.


A) σ\sigma E; σ\sigma F
B) σ\sigma K; σ\sigma F
C) σ\sigma F; σ\sigma K
D) σ\sigma F; σ\sigma E

E) All of the above
F) None of the above

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Riboswitches regulate transcription,rather than translation,in cells.

A) True
B) False

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The site on the DNA to which a repressor protein binds is the __________.


A) operon
B) operator
C) promoter
D) regulator

E) A) and B)
F) None of the above

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When a repressor binds to the operator site on the DNA it normally


A) inhibits the initiation of transcription.
B) reduces the rate of transcription.
C) terminates transcription that has already been initiated.
D) has no effect on transcription.

E) C) and D)
F) None of the above

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Small RNAs that regulate translation by binding to the leader region of mRNAs are called antisense RNAs.

A) True
B) False

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